Recombinant D-amino Acid Oxidase with Improved Properties
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چکیده
D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) was overproduced in Escherichia coli cells, and properties of the recombinant enzyme were studied. Single point mutants of the enzyme with 2.4-fold higher thermal stability or changed spectra of substrate specificity compared with wild-type enzyme were prepared. It was shown that mutant TvDAAO has higher catalytic efficiency in cephalosporin C oxidation in comparison with wild-type enzyme. One mutant of recombinant TvDAAO was crystallized and its structure was solved with resolution 2.8 Å.
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تاریخ انتشار 2008